Genetic Analysis Reveals a Major Effect QTL Associated with High Grain Zinc Content in Rice (Oryza sativa L.)

• 발행기관: 한국육종학회
• 저자명: Shaikh J. Mohiuddin Md. Ashraful Haque Md. Manjurul Haque Md. Tofazzal Islam Partha S. Biswas
• 간행물 정보: 『Plant breeding and biotechnology』Vol.8 No.4, 327~340쪽, 전체 14쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2020.12.01

영문 초록

Molecular mapping and application of quantitative trait loci (QTL) associated with a higher level of grain Zinc is a viable option to enhance zinc content in rice through breeding. An F2 population derived from a cross between a high yielding variety, BRRI dhan28, and a locally adapted Zn enriched cultivar, Kalobokri was used to map QTLs associated with higher levels of Zn in rice grain. The F2:3 progenies varied significantly (P ＜ 0.0001) in Zinc contents with a mean value remarkably higher than those in the superior parent. Through marker by trait analysis using IciMapping, we detected a large-effect QTL, qGZn3 on chromosome 3 between RM5419 and RM1164 spanning 1.83 Mb interval at the 0.05 level of significance with a threshold LOD of 10.61. This QTL showed a 21.1% (R² value) contribution to the total phenotypic variation for zinc content in the unpolished rice grains with 4.68 μg/g additive effect of Kalobokri alleles. We also detected 11 metal homeostasis related genes within the interval of qGZn3. In-silico analysis showed that four expressed sequence tags of one candidate gene (LOC_Os03g22810) encoding Cu/Zn superoxide dismutase, a metal-binding protein, are highly active in the endosperm as well as in the embryonic tissue of rice grain. Taken together, our results suggest that qGZn3 is a major QTL associated with high grain Zn content in the F2 progenies of rice. Our findings offer valuable genetic resources to facilitate breeding for high yielding and Zinc-enriched rice variety.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

AUTHOR CONTRIBUTIONS

ACKNOWLEDGEMENTS

ACKNOWLEDGEMENTS

REFERENCES

키워드

Biofortification Quantitative trait loci Selective genotyping Simple sequence repeat Zinc homeostasis related genes