GC 및 HPLC 비교분석에 기초한 차조기 종실내 tocotrienol 부재의 평가

• 영문명: Absence of Tocotrienol Form of Vitamin E in Purple Perilla (Perilla frutescens var. acuta Kudo) Seeds Confirmed by Comparative Analysis Using HPLC and GC
• 발행기관: 한국작물학회
• 저자명: 이영상(Young-Sang Lee) 김민경(Min-Kyoung Kim)
• 간행물 정보: 『한국작물학회지』제53권 제s호, 115~120쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.12.30

영문 초록

Lipid soluble vitamin E consists of toco-pherols and tocotrienols depending upon double bonds in phytyl side chains attached to chromanol ring. Recent reports on antioxidative, anticancer, and cholesterol-lowering effects of tocotrienols have increased researches and commerciali-zation of tocotrienols. Purple perilla (Perilla frutescens var. acuta Kudo) has been reported as a plant containing toco-trienols along with tocopherol forms of vitamin E based upon normal phase HPLC analysis. To confirm the existence or absence of tocotrienol form of vitamin E in purple perilla, comparative analysis using HPLC, GC/FID, and GC/MSD has been conducted for 14 purple perilla genetic accessions collected from Korea and Japan. Normal phase HPLC analysis showed α-, β-, γ-, and δ-tocopherols along with peaks with retention times quite similar to β- and γ-tocotrienols. Same purple perilla samples, analysed by GC exhibited α-, β-, γ-, and δ-tocopherols quantitatively equivalent to HPLC results. However, no peaks for β- and γ-tocotrienols could be observed and unknown two peaks of similar retention times with β- and γ-tocotrienols were identified not corres-ponding tocotrienols by GC/MSD. These results suggest the absence of tocotrienol form of vitamin E in purple perilla as well as the necessity of using GC-based quali-tative and quantitative vitamin E analysis to avoid misinter-pretation of peaks with similar retention times as tocotrie-nol isomers when analysed by an HPLC.

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GC HPLC purple perilla tocopherol tocotrienol