Kunitz Trypsin Inhibitor 발현 억제에 의한 콩 뿌리혹 수의 감소

• 영문명: Inhibition of SKTI Synthesis in Agrobacterium rhizogenes-induced Hairy Root Reduces the Number of Nodule in Soybean
• 발행기관: 한국작물학회
• 저자명: 김선형(Sun Hyung Kim) 임채우(Chae woo Lim) 박지영(Ji Young Park) 황철호(Cheol Ho Hwang)
• 간행물 정보: 『한국작물학회지』제54권 제3호, 299~306쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2009.09.30

영문 초록

In nitrogen-limited conditions, rhizobia lead to formation of nitrogen-fixing nodules on the roots of leguminous plants. The process of nodulation is autoregulated by pre-existing nodules in the same root system. The altered profile of sap proteins by inoculation with B. japonicum may indicate presence of a signal responsible for autoregulation transferred through stem. The 20 kDa protein enhanced by innoculation significantly decreased in intensity from 2.5 to 7 days after inoculation (DAI). However 6 kDa protein did increase during such a transition period. Western blot analysis showed that both 20 kDa and 6 kDa were cross-reacted with the SKTI antiserum. This suggests that SKTI may be involved in soybean nodulation by specific induction and degradation in stem sap during early stage of nodulation. RNAi technique and Agrobacterium rhizogenes-mediated transformation were applied to investigate the function of SKTI in nodulation. We have found that the number of rhizobium-induced nodule was much less in SKTIi-silenced hairy roots than the non-silenced. Indeed the quantitative RT-PCR showed that the expression level of SKTI gene was reduced over 40% in the transgenic hairy roots com-pared to the non-transgenic. It appears that the observed early induction of SKTI and degradation into small peptide in a specific time manner may be involved in autoregula-tion of nodulation in soybean and the specific mechanism of such regulation remains to be investigated.

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nodulation Kunitz trypsin inhibitor SKTI Hairy root transformation soybean