Influence of Antibiotics on Shoot Regeneration and Agrobacteium Suppression Using Cotyledonary Node in Korean Soybean Cultivars

• 발행기관: 한국작물학회
• 저자명: Kyung-Hee Kim Jae-Eun Lee Young-Up Kwon Byung-Moo Lee
• 간행물 정보: 『한국작물학회지』제54권 제3호, 307~313쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2009.09.30

영문 초록

Mature dry seeds of Korean cultivars, Daepung-kong, Muhankong, Myeongjunamulkong, Somyeongkong, Sowonkong, Jinpumkong, and Pungsannamulkong were used. The influence of antibiotics on elimination of Agrobacterium growth and shoot regeneration was estimated with cotyledo-nary node. Cefotaxime and timentin at the concentration of 250 and 500 mg/l suppressed Agrobacterium, especially cefo-taxime was an efficient antibiotic to suppress Agrobacterium in all cultivars. While carbenicillin and timentin at the con-centration of 50 and 100 mg/l were not sufficient to control the development of Agrobacterium, respectively. Cefotaxime and timentin represented high shoot formation rates com-pared with carbenicillin. Carbenicillin at low concentrations did not effectively suppress Agrobacterium and also had no effect on shoot development. Cefotaxime at the concentra-tion of 250 mg/l showed maximum frequency of shoot regenera-tion in cvs. Somyeongkong and Sowonkong. Furthermore, on medium containing cefotaxime, shoot was more quickly formed than the other antibiotics. The use of cefotaxime was very useful for elimination of Agrobacterium growth with cotyledonary node of Korean soybean cultivars.

목차

MATERIALS AND METHODS

RESULTS AND DISCUSSION

ACKNOWLEDGEMENT

REFERENCES

키워드

antibiotics soybean cotyledonary node regeneration Agrobacterium suppression