A Genome-wide Approach for Functional Analysis Using Rice Mutant

• 발행기관: 한국작물학회
• 저자명: Won Cheol Yim Dong Sub Kim Jun-Cheol Moon Cheol Seong Jang Byung-Moo Le
• 간행물 정보: 『한국작물학회지』제54권 제3호, 332~338쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2009.09.30

영문 초록

Rapid extension of genomic database leads to the remarkable advance of functional genomics. This study proposes a novel methodology of functional analysis using 5-methyltrytophan (5 MT) mutant together with their 2-DE analysis and public microarray database. A total of 24 proteins was changed in 5 MT mutant and four remarkably different expressed proteins were identified. Among them, three spots were converted to Affymetrix probe. A total of 155 microarray samples from Gene Expression Omnibus (GEO) in NCBI was retrieved and followed by constructing gene co-expression networks over a broad range of bio-logical issues through Self-Organising Tree Algorithm. Three co-expressing gene clusters were retrieved and each func-tional categorization with differential expression pattern was exhibited from 5 MT resistance mutant rice. It was indicated new co-expression networks in the mutant. This study suggests that on investigating possibility which cor-respond 2-DE to microarray database with their full potential.

목차

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENT

REFERENCES

키워드

rice (Oryza sativa) 5 MT 2-DE genome-wide mutant proteomic GEO clustering analysis