납이 HL 60 세포 내 Myo-inositol 수송 체계에 미치는 영향

• 영문명: Effect of Lead on the Myo-inositol Transport System in HL 60 Cell Line
• 발행기관: 한국환경보건학회
• 저자명: 정명규(Myung Kiu Chung)
• 간행물 정보: 『1. 한국환경보건학회지』제25권 제2호, 129~134쪽, 전체 6쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1999.06.30

영문 초록

Lead is the most ubiquitous toxic metal and released to the environment from natural and man-made sources. Presence of lead in the environment has led to an increasing awareness and concern of deterimental effect on ecosystem and human health. But there are several limits for toxicity assessment and protection human from toxicity of lead because the toxic effects of lead are detectable in practice only at high exposure concentraion. Therfore, it is very important to search sensitive biomakers at low exposure concentration which could not be observed any practical symptoms such as anemia, encephalopathy, and neuropathy in human. In the present study, to investigate myo-inositol concentration in cell could be a biomaker for lead cytotoxicity, the toxic effects of lead on myo-inositol uptake system were studied in the HL 60 cell line from three groups : normal control, lOppm-lead treated group, 80 ppm-lead treated group. Lead significantly reduced specific myo-inositol uptake in lead-treated groups, but did not affected nonspecific uptake. The magnitude of reduction in myo-inositol uptake was directly propotional to exposure dose. But myo-inositol uptakes in lead treated groups were significantly normalized by addition sodium ion to culture medium. These results suggest that myo-inositol concentration in the cell could be a useful biomaker in toxicity test with cell line and the toxic mechanism of lead on HL 60 cell might be a reduction in myo-inositol uptake, which induced by the perturbation of the sodiumion metabolism.

목차

I. 서론

II. 재료 및 방법

III. 결과 및 고찰

IV. 요약 및 결론

키워드

Myo-inositol Lead HL 60 cell line Specific uptake Biomaker