PCR 기법을 이용한 간편한 DNA Size Marker 제작 방법

• 영문명: A Simple PCR-Based Method for the Preparation of DNA Size Markers
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 박성환(Seong-Whan Park)
• 간행물 정보: 『산업기술연구논문지』산업기술연구논문지 제26권 1호, 9~14쪽, 전체 6쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2021.03.31

영문 초록

DNA size markers are widely used in molecular biology laboratories as DNA molecular weight standards. In this paper, I report a simple and convenient method for the preparation of DNA ladders using PCR technique. DNA ladders usually range from 100 bp to 10 kb, and many such 100 bp and 1 kb DNA ladders are commercially available. Conventionally, 100 bp to 5 kb DNA fragments were amplified using standard PCR with Taq DNA polymerase, and the large DNA fragments exceeding 5 kb were amplified using long-range PCR with a special thermostable polymerase. In this study, several types of DNA ladders were prepared by mixing the amplified DNA fragments in different combinations, which can be used as DNA size standards in agarose gel electrophoresis.

목차

Ⅰ. 서 론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험 결과

Ⅳ. 고 찰

참고문헌

키워드

중합효소연쇄반응 DNA 크기 표준 DNA 래더 프라이머 설계 아가로스 겔 전기영동 PCR DNA Size Standard DNA Ladder Primer Design Agarose Gel Electrophoresis