Anti-inflammatory activity of various seaweeds in LPS-PG stimulated HGF-1 cells

• 발행기관: 한국구강보건과학회
• 저자명: 윤현서(Hyun-Seo Yoon) 김혜진(Hye Jin Kim) 박충무(Chung-Mu Park)
• 간행물 정보: 『한국구강보건과학회지』제8권 제4호, 25~30쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2020.12.30

영문 초록

Objectives: Many seaweeds are considered important natural resources since their abundant bioactive compounds. The present study tried to analyze the anti-inflammatory activity of several seaweeds in the human gingival fibroblast (HGF)-1 cells. Methods: Lipopolysaccharide from Porphyromonas gingivalis (LPS-PG), one of the main causes in the progression of periodontal inflammation, was applied to induce inflammatory response in HGF-1 cells. Nitric oxide (NO) was used as one of the hallmarks for inflammation, which was analyzed by the Griess reaction. The protein expression level of inducible NO synthase (iNOS) was identified by the Western blot analysis. Results: Inhibitory effect of NO production was investigated in the ethanol extracts of Asparagopsis taxiformis Trevisan de Saint-Léon (ATEE), Distromium decumbens (Okamura) Levring (DDEE), Galaxaura fastigiata (GFEE), Hypnea japonica Tanaka (HJEE), Myelophycus simplex (Harvey) Papenfuss (MSEE), and Scytosiphon canaliculatus (Setchell & N.L. Gardner) Kogame (SCEE) in LPS-PG stimulated HGF-1 cells. As a result, ATEE and HJEE the most potently inhibited while GFEE and SCEE slightly ameliorated NO production without any cytotoxicity. But, DDEE and MSEE did not exhibit the extraordinary NO inhibitory effect in LPS-PG induced HGF-1 cells. Western blot analysis was also conducted to identify the inhibitory effect of both extracts, ATEE and HJEE that sharply attenuated NO production, in LPS-PG induced HGF-1 cells. ATEE and HJEE mitigated iNOS expression in a dose dependent manner, which was in accordance with the result of NO production. Conclusions: ATEE and HJEE in the algal extracts used in this study drastically reduced the LPS-PG-induced inflammatory response in HGF-1 cells. All of this ATEE and HJEE will be used as candidate substances for treatment and prevention of periodontal disease.

목차

Ⅰ. Introduction

Ⅱ. Materials and Methods

Ⅲ. Results and Discussion

REFERENCES

키워드

Human gingival fibroblast Lipopolysaccharide Nitric oxide synthase Porphyromonas gingivalis Seaweed