Analysis of Scutellaria baicaleinsis Georgi (Scutellariae Radix) by LC-DAD and LC-ESI/MS

• 발행기관: 한국자원식물학회
• 저자명: Youngbob Yu Pil-Son Choi Sungtae Koo Suhwan Chang
• 간행물 정보: 『한국자원식물학회지』제31권 제6호, 652~659쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.12.30

영문 초록

In this study, baicalin, as a marker substance of Scutellariae Radix, was quantitatively analyzed by a highperformance liquid chromatography-photodiode array detector (HPLC-DAD). We identified wogonoside, baicalein, andwogonin in the Scutellariae Radix by a high performance liquid chromatography-electrospray ionization-mass spectrometer(HPLC-ESI-MS). The baicalin was separated on a Xterra C18 column (5 ㎛, 4.6 x 250 ㎜) using mobile phase consisting of38% acetonitrile in 0.68% phosphoric acid. The baicalin spectrum in the Scutellariae Radix extracts was coincided bycomparing with UV-visible spectrum (200-550 ㎚) of baicalin standard in the library. The amount of baicalin in ScutellariaeRadix was 10.46%, which is higher than KFDA’s guideline. The marker substances of Scutellariae Radix showed a strongbase peak [M]+ in the positive detection mode following as; baicalin (m/z; 271 [MH+-sugar]+, 447 [M+H]+), wogonoside(m/z; 285 [MH+-sugar]+, 461 [M+H]+), baicalein (m/z; 271 [M+H]+), wogonin (m/z; 285 [M+H]+). These results areconsistent with the fragment pattern and molecular weight of standard components from literature.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgements

References

키워드

4 marker substances LC-DAD LC-ESI/MS Scutellariae Radix