A Successful Regeneration from Shoot Tips of Chrysanthemum morifolium (Ramat.) following Cryopreservation by Droplet-vitrification

• 발행기관: 한국자원식물학회
• 저자명: Jung-Yoon Yi Kotnala Balaraju Hyung-Jin Baek Mun-Seop Yoon Haeng-Hoon Kim Young-Yi Lee
• 간행물 정보: 『한국자원식물학회지』제31권 제6호, 675~683쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.12.30

영문 초록

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoottips of Chrysanthemum morifolium (Ramat.) cvs. ‘Borami’ and ‘Yes morning’. The shoot tips of Chrysanthemum wereprecultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7 M). Precultured explantswere treated with loading solution (LS, C6) containing glycerol 20% and sucrose 20% for 30 min and exposed todehydration solution (B5) containing 40% of glycerol and 40% of sucrose for 60 min at 25℃, and then transferred ontodroplets containing 2.5 ㎕ PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen(LN) for 1 h. The highest regeneration rate (%) was obtained when shoot tips were precultured with treatment-2 (exposingof shoot tips to MS + 0.3M sucrose for 30 h and then treated with MS+0.5 M sucrose for 16 h) at 25℃ in both the cultivars. The viability of cooled samples, followed by culturing on NH4NO3-free MS medium for first 5 days was increased totwo-fold (80.7%) regrowth rate over those cultured on normal MS medium or MS medium containing plant growthregulators. This result shows droplet-vitrification would be a promising method for cryobanking chrysanthemumgermplasm.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgements

References

키워드

Chrysanthemum Cryopreservation Liquid nitrogen Regrowth Vitrification