CSⅡ 약침의 포유류 배양세포 염색체이상 시험

• 영문명: In vitro Mammalian Chromosomal Aberration Test of CSⅡ-YAKCHIM
• 발행기관: 임상약침학회
• 저자명: 양재원(Jae-Won Yang) 안덕근(Duck-Gun An)
• 간행물 정보: 『임상약침학회학회지』대한면역약침학회지 제4권 제1호, 25~31쪽, 전체 7쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2015.06.30

영문 초록

Objectives : This study evaluated the potential related CSⅡ-YAKCHIM on chromosomal aberrations in Chinese Hamster Lung (CHL/IU) cells to provide the safety evidence of CSⅡ-YAKCHIM. Methods : In order to determine the high dose levels of the main study, the cell growth inhibition study was conducted. The high dose was selected at 5,000 μg/mL and it was sequentially diluted to produce lower dose levels of 39.1, 78.1, 156, 313, 625, 1,250 and 2,500 μg/mL. As a result, the cell growth inhibition was below 50% at all dose levels when compared with negative control value in the short time treatments without and with metabolic activation and the continuous treatment without metabolic activation. Therefore, the dose levels of the main study were selected as 1,250, 2500, and 5,000 μg/mL (short time treatment and continuous treatment). Also, the negative and positive control groups were used. Results : As a result of the main study, the frequency of cells with chromosome aberrations was less than 5% in the short time treatments without and with metabolic activation and the continuous treatment without metabolic activation. Also, there was no statistically significant differences when compared to that in the negative control group. In the positive control group, the frequency of cells with structural chromosomal aberrations was more than 10% and statistically significantly increased compared to that in the negative control group. Conclusions : Based on the results of this study, CSⅡ-YAKCHIM did not exhibited the chromosome aberrations under the conditions of this study.

목차

Ⅰ. 서 론

Ⅱ. 재료 및 방법

Ⅲ. 결 과

Ⅳ. 고찰 및 결론

참고문헌

키워드

CSⅡ-YAKCHIM pharmacopuncture toxicity chromosome aberration Chinese Hamster Lung Cell