학술논문
Development and characterization of mouse small intestinal enteroids
이용수 9
- 영문명
- 발행기관
- 한국예방수의학회
- 저자명
- Siu Lee Na-Yeon Gu Se-A Lee Jienny Lee Yoon-Hee Lee Bang-Hun Hyun
- 간행물 정보
- 『Journal of Preventive Veterinary Medicine』Vol.44, No.2, 62~68쪽, 전체 7쪽
- 주제분류
- 의약학 > 의학일반
- 파일형태
- 발행일자
- 2020.06.30
4,000원
구매일시로부터 72시간 이내에 다운로드 가능합니다.
이 학술논문 정보는 (주)교보문고와 각 발행기관 사이에 저작물 이용 계약이 체결된 것으로, 교보문고를 통해 제공되고 있습니다.
국문 초록
영문 초록
The culture of the intestinal epithelium into three dimensional (3D) structures typically termed organoid culture. Organoid culture is based on the ability of intestinal stem cells (ISCs), at the base of the crypt, perpetually to divide and produce a fully differentiated, polarized epithelium. Leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5) positive ISCs isolated from the intestine can form organoids in long-term culture. Thus, when cultured under the appropriate 3D conditions, single Lgr5⁺ ISCs undergo cycles of self-renewal, differentiation and morphogenesis, and self-organize into crypt-villus domains that house cycling ISCs and differentiated intestinal epithelial cells (IECs). In this study, we performed isolation, characterization and consecutive subculture of small intestinal crypts from BALB/c-nude mouse. Briefly, isolated mouse crypts were embedded in matrigel, cast into 40 μL droplets at the bottom of well in a 48-well plate. Following polymerization, the gels were overlaid with ISCs expansion medium containing B27, N2, N-acetylcysteine, epidermal growth factor, noggin, and R-spondin 1. As a result, mouse crypt-derived ISCs had enteroids and spheroid morphologies. We also confirmed by quantitative real-time RT-PCR that expression of ISCs-related specific genes (Lgr5, sox9) and IECs-related specific genes (chromogranin A, defensin-5, mucin-1, mucin-2, and villin) was maintained at eight passages or more. Thus, we observed that expression of specific markers and consecutive self-renewing in the mouse small intestinal crypt-derived organoids.
목차
서 론
재료 및 방법
결 과
고 찰
감사의 글
REFERENCES
해당간행물 수록 논문
- Poultry farm vehicle movements as risk factors for the 2014/15 highly pathogenic avian influenza epidemics in the Republic of Korea
- Development and characterization of mouse small intestinal enteroids
- Electrical stimulation promotes the differentiation of rabbit mesenchymal stem cells toward chondrocytes
- Protective effects of Achyranthes Radix root extract against CDDP-induced liver and kidney damage in SD rats: Application of big data analysis to traditional medicine
- Species distribution modeling for wild boar (Sus scropa) in the Republic of Korea using MODIS data
- Identification and antimicrobial susceptibility of Escherichia coli isolated from broiler chickens affected by colibacillosis in Tiaret Province
- Cytotoxicity of natural killer cells co-cultured with γ-irradiatedor mitomycin C-treated feeder cells
- Production of VP2 protein of canine parvovirus-2c expressed in baculovirus expression system
참고문헌
교보eBook 첫 방문을 환영 합니다!
신규가입 혜택 지급이 완료 되었습니다.
바로 사용 가능한 교보e캐시 1,000원 (유효기간 7일)
지금 바로 교보eBook의 다양한 콘텐츠를 이용해 보세요!