Production of VP2 protein of canine parvovirus-2c expressed in baculovirus expression system

• 발행기관: 한국예방수의학회
• 저자명: Seyeon Park Ju-Yeon Lee Jae Young Song Yoon-Hee Lee Hyeonhae Choi Seo Young Moon Min Ji Kim Suyeong Yun Soo-dong Cho Jienny Lee Bang-hun Hyun In-ohk Ouh
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.44, No.2, 96~98쪽, 전체 3쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2020.06.30

영문 초록

Viral protein 2 (VP2), which is the structural protein of parvovirus, can produce virus-like particles (VLPs) by a self-assembly process in vitro, making VLPs attractive vaccine candidates. VP2 of canine parvovirus (CPV) is responsible for neutralizing antibodies in immunized animals. In this study, VP2 protein of canine parvovirus-2c was expressed using a baculovirus expression system and assembled into parvovirus-like particles in insect cells. The results show that VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and a high hemagglutination (HA) titer (1:2⁷). The recombinant 6-His-tagged VP2 protein with a molecular mass of about 65 kDa was detected by anti-His antibody and anti-PPV serum. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of CPV and in the vaccination against CPV.

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키워드

Canine parvovirus CPV2-c expression purification VP2 protein