재조합 단백질의 생산성에 대한 저온 배양의 영향 고찰

• 영문명: Impact of Mild Hypothermic Culture on the Productivity of Recombinant Protein
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 문무상(Moo-Sang Moon)
• 간행물 정보: 『산업기술연구논문지』산업기술연구논문지 제24권 4호, 121~127쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

Mammalian cells producing recombinant proteins were typically cultured at the physiological temperature of 37℃. However, subphysiological culture of CHO cells suppressed cell growth, resulting in decrease of recombinant protein productivity in comparison to standard batch maintained at 37℃. At low temperature, cells were arrested in the G1 stage of the cell cycle and extended periods of their viability, enabling an extended production phase. This would greatly contribute to a more economic industrial process for the production of recombinant proteins. Meanwhile many studies reported that hypothermic cultures on recombinant protein production in rCHO cells were both cell-specific and product-specific. Although the mechanisms regulating these phenotypes during hypothermic culture are pooly understood, these data could be useful for future approaches to enhance the production of recombinant protein in rCHO cells.

목차

Ⅰ. 서 론

Ⅱ. 재조합 단백질의 생산성 향상을 위한 배양조건 최적화

Ⅲ. 고찰 및 결론

키워드

재조합 세포증식 중국 햄스터 난소세포 저온배양 생산성 Recombinant Cell Growth CHO Cell Hypothermic Culture Productivity