이질배수성 게놈 돌산갓의 복이배체(4n) 식물 생산

• 영문명: Insight on Doubled Haploid Production with an Amphidiploid Species ‘Dolsangat’ in Brassica Juncea
• 발행기관: 한국육종학회
• 저자명: 김정선(Jung Sun Kim) 서미숙(Mi-Suk Seo) 문미선(Mi-Sun Moon) 원소윤(So Youn Won) 권수진(Soo-Jin Kwon)
• 간행물 정보: 『한국육종학회지』Vol.51 No.4, 341~350쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.11.30

영문 초록

Amphidiploid Brassica juncea (AABB, 2n=36) contains the synthesized genome of the diploid ancestors of Brassica rapa (AA, 2n=20) and Brassica nigra (BB, 2n=16), proven the ‘triangle of U’ model. Varieties of the B. juncea include vegetables, oilseed crops, and medicinal plants in South Asia, China, and other regions. ‘Dolsangat’, one of the cultivars of B. juncea is widely used as the main ingredient for ‘KatKimchi’, a kind of Korean traditional food Kimchi. To develop an efficient polyploidization protocol of B. juncea, we used twenty accessions. Among them, we could induce the amphidiploid plants with 0.23% in natural. A successful of polyploidization, it is essential of chromosome doubling regent treatment of B. juncea. At first, we tried to colchicine treatment in the embryo stage and it was very harmful to the embryo and could get few plants. The second, we made the regeneration plants from embryo to rooting phase and shocked them in 0.34% colchicine contained distilled water. We could induce amphidiploid plants with a success rate of 63.4%. Also, we surveyed glucosinolate content and JB1, Alsami, and JD6 showed high total contents. These plants will use for genetic materials for breeding, genetic and molecular studies.

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키워드

Amphidiploid Brassica juncea Dolsangat microspore culture colchicine treatment