Agrobacterium을 이용한 제초제 저항성 옥수수 형질전환체 생산

• 영문명: Production of Transgenic Maize Plants with Herbicide Resistance Through Agrobacterium-mediated Transformation
• 발행기관: 한국육종학회
• 저자명: 홍준기(Joon Ki Hong) 이강섭(Gang-Seob Lee) 박기진(Ki Jin Park) 김주곤(Ju-Kon Kim) 장희정(Hee Jeung Jang) 서은정(Eun Jung Suh) 김경환(Kyung-Hwan Kim) 이연희(Yeon-Hee Lee)
• 간행물 정보: 『한국육종학회지』Vol.51 No.4, 290~297쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.11.30

영문 초록

Maize is the most important grain crop in the world. Genetic engineering technology has been used to enhance its various agronomical traits. The transformation of maize is a crucial step in the application of gene technologies to improve maize. The choice of genotype and explant material influences the transformation efficiency and the production of stable transgenic plants. Immature embryos of Hi IIA were infected with Agrobacterium tumefaciens LBA4404 including superbinary vectors (bar and GUS or GFP genes). The transformation efficiency was based on transgenic calli induction from immature embryos on the selection medium with 3 mg/L bialaphos. The transformation efficiency varied from 1.01 to 2.74%. The integration and expression of bar, GUS, and GFP genes were confirmed in T0 and T1 generations of transgenic plants using genomic PCR and the bar strip test. In addition, herbicide resistance in T1 transgenic plants was observed when leaves and whole plants were treated with Basta. These results suggest that the successful Agrobacterium-mediated transformation of Hi IIA will improve further opportunities for functional genomic and genome editing studies in maize.

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키워드

Genotype Herbicide resistance Immature embryo Maize Transformation