방사선 유도 내염성 증진 사료용 옥수수 돌연변이체 특성 분석

• 영문명: Characterization of a Gamma Radiation-Induced Salt-Tolerant Silage Maize Mutant
• 발행기관: 한국육종학회
• 저자명: 조철오(Chuloh Cho) 김경화(Kyung Hwa Kim) 최만수(Man-Soo Choi) 전재범(Jaebuhm Chun) 서미숙(Mi-Suk Seo) 정남희(Namhee Jeong) 진민아(Mina Jin) 손범영(Beom-Young Son) 김둘이(Dool-Yi Kim)
• 간행물 정보: 『한국육종학회지』Vol.51 No.4, 318~325쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.11.30

영문 초록

Salt stress is a significant factor limiting growth and productivity in crops. However, little is known about the response and resistance mechanism to salt stress in maize. The objective of this research was to develop an enhanced salt tolerant silage maize by mutagenesis with gamma radiation. To generate gamma radiation-induced salt-tolerant silage maize, we irradiated a KS140 inbred line with 100 Gy gamma rays. Salt tolerance was determined by evaluating plant growth, morphological changes, and gene expression under NaCl stress. We screened 10 salt-tolerant maize inbred lines from 2,248 M2 mutant populations and selected a line showing better growth under salt stress conditions. The selected 140RS516 mutant exhibited improved seed germination and plant growth when compared with the wild-type under salt stress conditions. Enhanced salt tolerance of the 140RS516 mutant was attributed to higher stomatal conductance and proline content. Using whole-genome re-sequencing analysis, a total of 328 single nucleotide polymorphisms and insertions or deletions were identified in the 140RS516 mutant. We found that the expression of the genes involved in salt stress tolerance, ABP9, CIPK21, and CIPK31, was increased by salt stress in the 140RS516 mutant. Our results suggest that the 140RS516 mutant induced by gamma rays could be a good material for developing cultivars with salt tolerance in maize.

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키워드

silage maize gamma rays mutagenesis salt tolerance