Real-time PCR을 이용한 환경 중 물 시료의 레지오넬라 분석법 연구

• 영문명: Study on the Enumeration of Legionella in Environmental Water Samples Using Real-time PCR
• 발행기관: 한국환경보건학회
• 저자명: 이정희(Jung-Hee Lee) 박명기(Myoung-Ki Park) 김윤성(Yun-Sung Kim) 윤희정(Hee-Jeong Yun) 이창희(Chang-Hee Lee) 정아용(Ah-Yong Jeong) 윤미혜(Mi-Hye Yoon)
• 간행물 정보: 『1. 한국환경보건학회지』제45권 제5호, 511~519쪽, 전체 9쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2019.10.30

영문 초록

Objectives: The standard method for the enumeration of environmental Legionella is culturing, which has several disadvantages, including long incubation and poor sensitivity. The purpose of this study is to demonstrate the usefulness of real-time PCR and to improve the standard method. Methods: In 200 environmental water samples, a real-time PCR and culture were conducted to detect and quantify Legionella. Using with the results of the survey, we compared the real-time PCR with the culture. Results: Each real-time PCR assay had 100% specificity and excellent sensitivity (5 GU/reaction). In the culture, 36 samples were positive and 164 samples were negative. Based on the results of the culture, real-time PCR showed a high negative predictive value of 99%, 35 samples were true positive, 105 samples were true negative, 59 samples were false positive and one sample was a false negative. Quantitative analysis of the two methods indicated a weak linear correlation (r²=0.29, r²=0.61, respectively). Conclusions: Although it is difficult to directly apply quantitative analysis results of real-time PCR in the enumeration of environmental Legionella, it can be used as a complementary means of culturing to rapidly screen negative samples and to improve the accuracy of diagnosis.

목차

I. 서 론

II. 재료 및 방법

III. 결 과

IV. 고 찰

V. 결 론

키워드

Culture environment Legionella real-time PCR water sample