제초제 저항성 형질전환 벼 계통의 농업적 특성과 분자생물학적 평가

• 영문명: Evaluation of agronomic and molecular biological characteristics of the herbicide resistance transgenic rice
• 발행기관: 한국육종학회
• 저자명: 박향미 김율호 서정필 최만수 김기종 신동범 박장환 이장용
• 간행물 정보: 『한국육종학회지』Vol.39 No.2, 148~154쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2007.06.30

영문 초록

Agronomic characteristics of transgenic herbicide-resistant rice lines were evaluated under field condition. Differences in agronomic traits and rice quality were observed between transgenic plants and the corresponding untransformed controls. Transgenic Ilpumbyeo lines flowered earlier than untransformed controls, whereas transgenic Nagdongbyeo flowered at the same time as untransformed control. With regard to the yield and rice quality, most of the transgenic lines showed lower yield than control except some selected transgenic lines. Selected Nagdongbyeo transgenic line also showed good eating quality comparable to the control although most selected Ilpumbyeo transgenic lines showed slightly lower eating quality and increased white center/belly in the rice grain. In order to find the main factor leading to somaclonal variation among transgenic lines, Tos17, a rice retrotransposon activated by prolonged tissue culture period was investigated. Although the transgenic lines carried only one or two of transformed bar gene, the copy number of Tos17 increased in most transgenic lines compared with control. The activation of Tos17 was not detected in selected promising transgenic lines such as ND115-15-1-B and IP23-3-3, suggesting that the increased copy number of Tos17 may have negatively affected agronomic characteristics of transgenic rice. Agronomic characteristics of transgenic herbicide-resistant rice lines were evaluated under field condition. Differences in agronomic traits and rice quality were observed between transgenic plants and the corresponding untransformed controls. Transgenic Ilpumbyeo lines flowered earlier than untransformed controls, whereas transgenic Nagdongbyeo flowered at the same time as untransformed control. With regard to the yield and rice quality, most of the transgenic lines showed lower yield than control except some selected transgenic lines. Selected Nagdongbyeo transgenic line also showed good eating quality comparable to the control although most selected Ilpumbyeo transgenic lines showed slightly lower eating quality and increased white center/belly in the rice grain. In order to find the main factor leading to somaclonal variation among transgenic lines, Tos17, a rice retrotransposon activated by prolonged tissue culture period was investigated. Although the transgenic lines carried only one or two of transformed bar gene, the copy number of Tos17 increased in most transgenic lines compared with control. The activation of Tos17 was not detected in selected promising transgenic lines such as ND115-15-1-B and IP23-3-3, suggesting that the increased copy number of Tos17 may have negatively affected agronomic characteristics of transgenic rice.

키워드

제초제 저항성 형질전환 벼 제초제저항성품종 잡초 Tos17 GM crop Yield Quality bar유전자 rice transformation bar herbicide resistance