Evaluation of cytoplasm types by PCR-marker in male-sterility of onion (allium cepa L.)

• 영문명: Evaluation of cytoplasm types by PCR-marker in male-sterility of onion (Allium cepa L.)
• 발행기관: 한국육종학회
• 저자명: Cheol-woo Kim Eui-tai Lee Hyun-ju Park Young-seok Jang In-hu Choi Jin-ki Bang Sung-ju Ahn Gap-chae Chung
• 간행물 정보: 『한국육종학회지』Vol.39 No.2, 201~205쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2007.06.30

영문 초록

The production of hybrid onion (Allium cepa L.) seed is economically feasible using systems of cytoplasmic-genic male sterility(CMS). The identification of cytoplasm types might be helpful to development of sterile and maintainer lines. Due to this reason, a knowledge of the cytoplasm type is essential for hybrid breeding in onion. Especially, due to the biennial generation time of onion, to distinguish of onion cytoplasm type in individual plant may be helpful to reduce the efforts in a breeding program, when new sterile lines and the corresponding maintainer lines be developed. In our study, we applied the PCR-marker (orfA-501) to identify the cytoplasmic genotypes of collected 100 accessions of bulb onion. Among accessions, S-cytoplasm was found in 57 accessions. Nineteen accessions possessed only N-cytoplasm and twenty four accessions possessed both S- and N-cytoplasm. The production of hybrid onion (Allium cepa L.) seed is economically feasible using systems of cytoplasmic-genic male sterility(CMS). The identification of cytoplasm types might be helpful to development of sterile and maintainer lines. Due to this reason, a knowledge of the cytoplasm type is essential for hybrid breeding in onion. Especially, due to the biennial generation time of onion, to distinguish of onion cytoplasm type in individual plant may be helpful to reduce the efforts in a breeding program, when new sterile lines and the corresponding maintainer lines be developed. In our study, we applied the PCR-marker (orfA-501) to identify the cytoplasmic genotypes of collected 100 accessions of bulb onion. Among accessions, S-cytoplasm was found in 57 accessions. Nineteen accessions possessed only N-cytoplasm and twenty four accessions possessed both S- and N-cytoplasm.

키워드

Allium cepa Onion Cytoplasmic male sterility Cytoplasm Polymerase chain reaction PCR Polymerase chain reaction (PCR)