우결핵의 역학조사를 위한 mycobacterium bovis의 분자생물학적 감별기법

• 영문명: Molecular typing of mycobacterium bovis for epidemiological investigation of bovine tuberculosis
• 발행기관: 한국예방수의학회
• 저자명: 방혜은(Hye Eun Bang) 이혜영(Hye Young Lee) 이진희(Jin Hee Lee) 조상래(Sang Nae Cho)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.22, No.2, 123~130쪽, 전체 8쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1998.05.30

영문 초록

The goal of this study was set to establish the molecular tools for typing of Mycobacterium bovis strains. Various M. bovis strains from different sources were typed by several typing methods including restriction fragment length polymorphism(RFLP) analysis and polymerase chain reaction(PCR). Probes for the RFLP analysis were derived from IS6110, IS1081, and direct repeat(DR) sequences. In addition, the PCR-based variable number tandem repeats(VNTR) typing was employed to differentiate M. bovis strains. Of nine M. bovis strains examined using RFLP and VNTR, seven M. bovis strains had different patterns, and two field isolates from Mexico were not distinguishable, thus indicating the same origin. The results from our study clearly show that M. bovis strains can be effectively typed by employing these molecular typing methods.

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Mycobacterium bovis Molecular typing RFLP PCR VNTR