설사환자로부터 Verotoxin 생산대장균의 분리 및 생물학적 독성

• 영문명: Survey on Isolation and Biological Cytotoxicity of Verotoxin-Producing Escherichia coli Isolated from Diarrheal Patients
• 발행기관: 한국예방수의학회
• 저자명: 진형근(Hyeung Keon Jin) 차인호(In Ho Cha) 김용환(Yong Hwan Kim)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.20, No.2, 89~96쪽, 전체 8쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1996.05.30

영문 초록

The biological characteristics, isolation and/or distibution ratio, cytotoxicity and serological factors were studied for verotoxin-producing Escherichia coli (VTEC) from 145 diarrheal patients in Pusan area. The isolation ratio of VTEC was 8.5% (15 strains of 177 strains), and 5.6% of diarrheal patients were harbouring VTEC. Cytotoxicity for culture supernatants of isolated strains was assayed to Vero cell and HeLa cell monolayers. One strain indicated CD₅₀ at a 10⁶ dilution, uncommonly. A total of 15 isolates produced cytotoxic activity to Vero cell and HeLa cell. When 10² CD₀ units were tested for neutralization with rabbit antiserum against VT1 or VT2, 12 of the 15 isolates were found to secrete VT2, 3 strains in combination with VT1. In addition, 3 isolates secreted only VT1. The serotypes of 15 VTEC were classified by 7 serotypes (O2, O4, O111, O113, O114, O115 and O118).

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Escherichia coli Verotoxin Cytotoxicity