輸入畜産物로 부터의 殘留 抗生物質 檢出을 위한 새로운 方法의 開發에 관한 硏究

• 영문명: Studies on the new method development for the detection of residual antibiotics from animal products, particulary attributed to the ampicillin of the β-lactam antibiotics
• 발행기관: 한국예방수의학회
• 저자명: Won Chang Lee(李元暢) Jong Bae Kim(金鐘培) Chi Ho Lee(李致鎬)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.18, No.2, 155~160쪽, 전체 6쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1994.06.30

영문 초록

Modern livstock and poultry farmining methods involve the use of a large number of different veterinary drugs, for both therapeutic and prophylatic purposes. This study is conducted to develop more convenient simultaneous determination by chemiluminescent method for the residue of ampicillin which is one of the β-Lactam antibiotics in the animal products. The results obtained are as followings : The titrated concentration of D-AA oxidase was 16.6 ㎍/ml, FDA(flavin adenine nucleotide) concentration showed the highest reaction at a 50㎍/ml. The titration concentration of peroxidase was 10 ㎍/ml, ABEI (aminobutyl ethyl isoluminal ) concentration showed the highest reaction at a 10 ㎍/ml. Sensitivity of D-Ala was 10 ㎍/ml. The titration concentration of DD-carboxypeptidase was 0.12 ㎍/ml, the substrate Ac₂-L-Lys-D-Ala-D-Ala showed the titrated concentration of 28 ㎍/10㎕. Ampicillin was detected at the levels of 1.0 ng/ml under these conditions.

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키워드

β-Lactam D-AA oxidase Peroxidase DD-carboxy-peptidase Ampicillin