조직중의 잔류 diethylstilbestrol과 zeranol의 동시분석법에 관한 연구

• 영문명: A study of residual diethylatibetrol and zeranol determinationin in tissues
• 발행기관: 한국예방수의학회
• 저자명: Jong Myung Park(朴鍾鳴) In Cheon Kim(金仁千) Young Hwa Sim(沈暎和) Sang Hee Jeong(鄭詳憙) Sun Namgoong(南宮琁) Keun Sik Park(朴根植) Mun Han Lee(李文漢) Chang Up Lee(李昌業)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.16, No.3, 249~256쪽, 전체 8쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1992.12.30

영문 초록

A simultaneous analysis method using GC/MS was developed to monitor residual DES and zeranol. The residues in tissues were determined by the method developed. The residues in tissues were homogenized with sodium acetate buffer, hydrolysed with β-glucuronidase and extracted with acetonitrile. Lipids in the extract were removed with dichloromethane, the solvent was evaporated and then the residues were redissolved in alcohol mixture. Sep-pak cartridge was applied for clean-up and the separated residues were derivatized using TMSI and BSTFA. The residues were determined by GC/MS with DB-5 column, helium as a carrier gas, electron impact ionization mode and TIC and SIM scan mode. The limit of detection was 1 ppb and the calibration curves showed good linearity.

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키워드

Zeranol Diethylsilbestrol GC/MS