토양으로부터 α-glucosidase 저해제 생산균의 분리 및 특성

• 영문명: Isolation and Characterization of Bacteria Producing α-Glucosidase Inhibitor from Soil
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 오광근(Kwang Keun Oh) 김연향(Yeon Hyang Kim) 박종일(Jong Il Park) 문무상(Moo Sang Moon)
• 간행물 정보: 『산업기술연구논문지』산업기술연구논문지 제23권 1호, 41~49쪽, 전체 9쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2018.03.30

영문 초록

The isolation, characterization and culture conditions of bacteria producing α-glucosidase inhibitor from soil and Korean fermented foods were investigated. As a result of screening for the α-glucosidase inhibitory activity on 100 bacteria isolated, strain N1-5 with an inhibition ratio of 71.6% against α-glucosidase activity compared with the inhibition ratio of acarbose(25μg/ml) of 49.1%, was finally selected. This strain was identified by Vitek2 compact system and 16S rDNA sequencing, and named Bacillus subtilis N1-5. The optimum carbon source of B. subtilis N1-5 for the production of α-glucosidase inhibitor was 1% glucose and the optimum nitrogen source was 0.6% polypeptone. The production of α-glucosidase inhibitor by B. subtilis N1-5 showed a typical primary metabolite production pattern in the 5-liter jar fermenter using optimized media. The partially purified α-glucosidase inhibitor by B. subtilis N1-5 was maintained at pH 2.0~3.0 without significantly changing α-glucosidase inhibitory activity. From the above results, it was confirmed that B. subtilis N1-5 isolated from soil produces a relatively stable α-glucosidase inhibitor similar to acarbose, a therapeutic agent for diabetes.

목차

ABSTRACT

Ⅰ. 서론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험결과 및 고찰

Ⅳ. 결론

참고문헌

키워드

α-글루코시다제 저해제 고초균 당뇨병 α-글루코시다제 저해활성 α-Glucosidase Inhibitor Bacillus Subtilis Diabetes α-Glucosidase Inhibitory Activity