Three Common Subunits in the Editing Domains of Class Ia tRNA Synthetases

• 발행기관: 강원대학교 산업기술연구소
• 저자명: Lee Keun Woo Kwon Yong Jung James M. Briggs
• 간행물 정보: 『산업기술연구』vol.24, 139~142쪽, 전체 4쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2004.09.30

영문 초록

To identify conserved structural or functional subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through sequence alignments of the CP1 domains, three conserved regions were found near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed that there exist three common structural subunits in all of the five editing RS structures. The new alignment suggests a translocation movement of the CP1 domain caused by the binding with tRNA. Based on the experimental and modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding.. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through all class Ia editing RSs.

목차

Abstract

1. Introduction

2. Modeling

3. Results

4. Conclusions

5. Acknowledgment

References

키워드

aminoacyl-tRNA synthetase(aaRS) editing domain modeling