천궁 현탁배양에서 체세포배 동조화, 저장 및 식물체 재분화

• 영문명: Synchronization, storage and regeneration of somatic embryos in suspension culture of ligusticum chuanxiong
• 발행기관: 한국육종학회
• 저자명: 박주현(Ju Hyun Park) 채영암(Young Am Chae)
• 간행물 정보: 『한국육종학회지』Vol.27 No.2, 163~169쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1995.06.30

영문 초록

This study was carried out to investigate the synchronization of somatic embryogenesis for rapid mass production of somatic embryos in suspension culture of Ligusticum chuanxiong. Suspension culture was better than solid culture for high frequency of somatic embryos. The optimum combination of inoculating cells and medium volume was 3㎖ of inoculating cells in 20㎖ of medium or 6㎖, in 40㎖, respectively. When spermidine was added, somatic embryo development was increased in concentration of 30㎎/ℓ but the frequency of abnormal embryos was also increased. Adding of spermine inhibited somatic embryo formation. ABA (0.05㎎/ℓ) treatment after twenty days of culture 20㎎/ℓ inabenfide treatment after three weeks of culture was the most effective for synchronization. Uniconazol treatment had no effect on synchronization of somatic embryo formation. Inositol (1g/ℓ) as osmotic stabilizer enhanced somatic embryo formation and synchronization, however, mannitol had no effect. Somatic embryos could be stored for 2~3 months long at 4℃.

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키워드

Ligusticum chuanxiong Synchronization of somatic embryo ABA Inabenfide Uniconazol Inositol Mannitol Polyamine