Erwinia carotovora 유래의 cellulase 유전자의 클로닝 및 대장균에서의 발현

• 영문명: Cloning and expression of cellulase genes from Erwinia carotovora in E. coli
• 발행기관: 강원대학교 산업기술연구소
• 저자명: 김세돈(Kim, Se-Don) 최신건(Choi, Shin-Geon)
• 간행물 정보: 『산업기술연구』vol.29, 121~126쪽, 전체 6쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2009.09.30

영문 초록

New cellulase genes, named as CelV2 and CelN1, respectively, were isolated from Erwinia carotovora ATCC15713 and expressed in E. coli. The CelV2 and CelN1 gene were PCR amplified with degenerated primers and PCR products were sequenced and expressed in E. coli. Two new cellulase genes showed 97% homologies with previously reported Erwinia cellulase genes. The recombinant cellulase were purified with Ni-NTA column chromatography and its enzymatic properties were characterized. The optimum temperature of two enzymes were about 50℃ degree and optimum pH were around pH7.0. The newly isolated celluase genes could be used for enhancing substrate range of alcohol-producing bacteria such as Zymomonas mobilis.

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키워드

Erwinia carotovora 셀룰레이스 유전자 클로닝 발현 Erwinia carotovora cellulase gene cloning expression