빙핵활성단백질의 N-terminal부분을 이용한 녹색형광단백질의 Zymomonasmobilis세포 표면 발현

• 영문명: Display of green fluorescentprotein (GFP)on the cell surface of Zymomonas mobilis using N-terminal domain of ice nucleation protein
• 발행기관: 강원대학교 산업기술연구소
• 저자명: 이은모(Lee,Eun-Mo ) 최신건(Choi,Shin-Geon)
• 간행물 정보: 『산업기술연구』vol.29, 115~120쪽, 전체 6쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2009.09.30

영문 초록

Green fluorescent protein (GFPuv) was displayed on the surface of ethanol-producing bacteria Zymomonas mobilis using N-terminaldomain of ice nucleation protein (INP) as an anchoring motif. To evaluate the ice nucleation protein as plausible anchor motif in Z. mobilis, GFPuv gene was subcloned into Zymomonas expression vector yielding pBBR1MCS-3/pPDC/INPN/GFPuv plasmid., INP-GFPuv fusion protein was expressed in Z.mobilis and its fluorescence was verified by confocal microscopy. The successful display of GFPuv on Zymomonas mobili ssuggest that INP anchor motif could be used for future fusion partner in Z. mobili sstrain improvement.

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키워드

Zymomonas mobilis 빙핵활성단백질 (INP) 녹색형광단백질 세포 표면 발현 Zymomonas mobilis ice nucleation protein (INP) green fluorescent protein cell surface display