Improvement of the Doplet-vitrification Method for the Cryopreservation of Cultivated Potato Shoot Tips

• 발행기관: 한국육종학회
• 저자명: Jung Yoon Yi Sok Young Lee Gi An Lee Jong Wook Jeong Ji Hong Cho Haeng Hoon Kim
• 간행물 정보: 『한국육종학회지』Vol.44 No.2, 94~99쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.06.30

영문 초록

Cryopreservation has been known as an efficient method for long-term preservation of clonally propagated plants, and several cryopreservation methods have been developed. Among them, a droplet-vitrification method for potato using axillary shoot tips in vitro has been established previously. In this study, we have optimized the procedure in which explants were submitted to a step-wise pre-culture in liquid sucrose-enriched medium (0.3 and 0.7 M for 7 and 17 h, respectively). The pre-cultured explants were dehydrated with PVS3 (w/v, 50% glycerol + 50% sucrose) for 90 min or modified PVS2 vitrification solution (w/v, 37.5% glycerol + 15% DMSO + 15.0% ethylene glycol + 22.5% sucrose) for 30 min. This two dehydration solutions produced post-cryopreservation regeneration percentages of 57.2% and 80.9%, respectively. We also compared a new post-culture medium (0.1 mg･L -1 GA 3 , 0.1 mg･L -1 kinetin) with the conventional one (0.15 mg･L -1 IAA, 0.2 mg･L -1 zeatin, 0.05 mg･L -1 GA 3 ); the shooting initiation rates were 80.9% and 43.5%, respectively. The results suggest that the modified droplet-vitrification protocol described in this study is more effective, easier to implement, and more economical than the droplet-vitrification protocols currently used for potato.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

REFERENCES

키워드

Potato Cryopreservation Shoot tip Regeneration rate Vitrification solution