대장균에서 돼지 생식기ㆍ 호흡기 증후군 바이러스 ORF 7 유전자의 발현

• 영문명: Expression of the PRRS Virus ORF 7 Gene in E. coli
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 장재선(Jae-Seon Jang)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제22권 2호, 39~45쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2017.06.30

영문 초록

As a preceding study for the development of a safe and new recombinant vaccine or a diagnostic kit against Porcine reproductive and respiratory syndrome (PRRS), we plan to produce an open reading frame 7 (ORF 7) protein of PRRS virus. To do this, we construct a pET-ORF 7 recombinant expression vector by cloning the ORF 7 gene into the pET expression vector. Then the expression vector pET-ORF 7 was transformed into Escherichia coli BL21/DE3 and expression was induced by Isopropyl β-D-1-thiogalactopyranoside (IPTG). The relative amount of the ORF 7 protein was analyzed by SDS-PAGE. The recombinant plasmid pET-ORF 7 was identified to be expressed successfully in E. coli. The molecular weight of the expression protein was approximate 14 kDa, The ORF 7 protein was detected at 30 min after IPTG induction and increased to 4 hours as time-dependent manner. We will utilize it for the preparation of monoclonal antibody necessary to the development of a diagnostic kit against PRRS virus.

목차

Ⅰ. 서 론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험결과

Ⅳ. 고찰

키워드

Porcine Reproductive and Respiratory Syndrome(PRRS, 돼지 생식기ㆍ 호흡기 증후군) PRRS Virus(돼지 생식기ㆍ 호흡기 증후군 바이러스) Open Reading Frame 7(ORF 7) Expression(발현) Isopropyl β-D-1-Thiogalactopyranoside(IPTG)