비타민E 투여가 Hamster 협낭점막의 9, 10-dimethyl-1, 2-benzanthracene(DMBA)유도 구강상피암 발암과정에 미치는 영향

• 영문명: Effects of vitamin E on the hamster buccal pouch carcinogenesis induced by 9, 10-dimethyl 1, 2-benzanthracene(DMBA)
• 발행기관: 대한구강악안면외과학회
• 저자명: 이철원(Cheol Won Lee)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제13권 제1호, 123~147쪽, 전체 25쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1987.06.30

영문 초록

The relationship of nutritional supplements and cancer is receiving renewed attention. Vitamin E (α-tocopherol) is accepted one of essential nutrients for man and thought to have antioxidant function and free radical scavenger action and etc. The present study was undertaken to investigate the effect of vitamin E on the hamster buccal pouch carcinogenesis induced by DMBA. 75 syrian golden hamster (Mesocricetus auratus), 6 weeks old, were used in this experiment and were divided into normal (5), control Ⅰ, Ⅱ (10), experimental group Ⅰ, Ⅱ, Ⅲ (60). In experimental group and control group, the left buccal pouch of hamster was painted three times a week with 0.5% DMBA in heavy mineral oil and only heavy mineral 03 respectively. But in experimental group Ⅱ and control group Ⅱ, animals received 10 IU of vitamin E twice a week on days alternate to the DMBA painting. In experimental group Ⅲ, animals received vitamin E also, but they received 4 weeks before DMBA painting in order to investigate the long term effect of vitamin E. All animals were sacrified serially at 8th, 10th, 12th, 14th, and 16th week. Buccal pouches were photographed and excised. Tumors in the left buccal pouches were measured and counted for statistical analysis. Excised tissues were examined light-and eclectromicroscopically. The results were as follows; 1. In experimental group Ⅱ, Ⅲ received vitamin E, there was a significant delay in tumor formation up to 12 weeks, and that from 12 to 16 weeks, there were fewer tumors and their average size was smaller than group Ⅰ. But there was no difference between group Ⅱ and Ⅲ. 2. In experimental group Ⅰ, mild dysplasia with hyperkeratosis and acanthosis was observed at 8 weeks, carcinoma-in-situ and dysplasia at 10 weeks, frankly invasive carcinoma at 12 weeks, extensive tumors with invasion and surface necrosis at 14,16 weeks. 3. In experimental group Ⅱ, hyperkeratosis and acanthosis was observed at 8 weeks, carcinomain-situ and mild dysplasia at 10 weeks, frankly invasive carcinoma at 12 weeks, invasive carcinoma and carcinoma-in-situ at 14,16 weeks. 4. In experimental group Ⅲ, mild dysplasia and hyperkeratosis was observed at 8 weeks, moderate dysplasia at 10 weeks, carcinoma-in-situ and dysplasia at 12 weeks, invasive carcinoma and carcinoma-in-situ at 14,16 weeks. 5. Electromicroscopic studies of developed tumors in each group observed moderately differentiated type of epidermoid carcinoma. They showed nuclear pleomorphism, clumping of tonofilaments, less frequent number of desmosomes widened intercellular spaces with microvillus-like structures and degenerated mitochondria. This results suggest that vitamin E has some inhibition effects on carcinogenesis but there was no long term effect of vitamin E.

목차

I. 서론

II. 실험재료 및 방법

III. 실험성적

IV. 총괄 및 고찰

V. 결론

참고문헌

Abstract