정상 치은 조직에서 배양된 각화세포에서 Cyclosporine A에 의한 MMP-1, TIMP-1, TGF-β1의 발현에 관한 연구

• 영문명: THE mRNA EXPRESSION OF MMP-1, TIMP-1, TGF-β1 IN GINGIVAL KERATOCYTES FROM GINGIVAL HYPERPLASIA INDUCED BY CYCLOSPORINE A
• 발행기관: 대한구강악안면외과학회
• 저자명: 강학수(Hag-Soo Kang) 이재선(Jae-Sun Lee) 빙정호(Jung-Ho Bing) 박창주(Chang-Joo Park) 임재중(Jae-Jung Im) 황경균(Kyung-Gyun Hwang) 심광섭(Kwang-Sup Shim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면회과학회지 제34권 제4호, 405~411쪽, 전체 7쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2008.08.10

영문 초록

Purpose : Cyclosporine A (CsA) is a versatile immunosuppresive agent used to prevent graft rejection syndrome and treat autoimmune disease. One of the major side effects associated with CsA is the abnormal gingival hyperplasia. The purpose of this study was to investigate the relationship between the mRNA expression of the MMP-1, TIMP-1, and TGF-β1 and the concentration of CsA in cultured human gingival keratinocytes. Materials & Methods : Gingival keratocytes were obtained from gingival tissues of 4 healthy donors. The cultured gingival keratocytes were incubated with increasing concentrations of CsA (0-2000 ng/ml) for 24 hours and the expression of MMP-1, TIMP-1, and TGF-β1 were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results : The expressions of MMP-1 and TGF-β1 were not significantly different according to the concentrations of CsA. The expression of TIMP-1 was significantly increased at the CsA concentration of 500 ng/ml. Conclusion : We concluded that the gingival hyperplasia induced by CsA was more related with TIMP-1 than MMP-1 or TGF-β1 on gingival collagen metabolism in patients treated with CsA.

키워드

MMP-1 TIMP-1 TGF-β1 cyclosporine A gingival keratinocyte