당뇨 유도 백서 경골에 매식한 티타늄 임플란트 주위에 지방조직 유래 줄기세포 적용 시 골치유에 미치는 영향

• 영문명: Effect of adipose-derived stem cells on bone healing on titanium implant in tibia of diabetes mellitus induced rats
• 발행기관: 대한구강악안면외과학회
• 저자명: 김민구(Min-Gu Kim) 정인교(In-Kyo Jung) 신상훈(Sang-Hun Shin) 김철훈(Chul-Hoon Kim) 김복주(Bok-Joo Kim) 김정한(Jung-Han Kim) 황영섭(Young-Sup Hwang) 정유진(Eu-Gene Jung) 김진우(Jin-Woo Kim) 김욱규(Uk-Kyu Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제36권 제5호, 392~401쪽, 전체 10쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2010.10.30

영문 초록

Introduction: Diabetes mellitus, as a major health problem for the elderly has been shown to alter the properties of the bone and impair bone healing around a titanium implant in both humans and animals. The aim of this study was to examine the effect of adipose-derived stem cells on the healing process around a titanium implant in streptozotocin-induced diabetic rats. Materials and Methods: Thirteen rats were divided into two groups: adipose-derived stem cells injected group and a control group. A titanium screw implant (diameter: 2.0 mm, length: 3.5 mm) was placed into both tibia of 13 rats: 13 right tibia as the control group and 13 left tibia as the experimental group. The rats were sacrificed at different intervals (1, 2, and 4 weeks) after implantation for histopathology observations and immunohistochemistric analysis. Results: The histopathological findings revealed earlier new formed bone in the experimental group than the control group. In particular, at 1 week after implantation, the experimental group showed more newly formed bone and collagen around the implant than the control group. In immunohistochemistric analysis, osteoprotegerin (OPG) expression in the experimental group increased early compared to that of the control group until 2 weeks after implantation. However, after 2 weeks, OPG expression in the experimental group was similar to OPG expression in the control group. The receptor activator of nuclear factor κB ligand (RANKL) expression in the experimental group increased early compared to that of the control group, and then decreased at 2 weeks. After 2 weeks, the level of RANKL expression was similar in both groups. Conclusion: These results suggest that adipose-derived stem cells in implantation can promote bone healing around titanium, particularly in diabetes mellitus induced animals.

키워드

Stem cell Dental implants Diabetes mellitus Rats