골막기원세포의 조골세포로의 분화과정에서 염증성 사이토카인의 효과

• 영문명: Evaluation of osteogenic activity of periosteal-derived cells treated with inflammatory cytokines
• 발행기관: 대한구강악안면외과학회
• 저자명: 박봉욱(Bong-Wook Park) 최문정(Mun-Jeoung Choi) 하영술(Young-Sool Hah) 조희영(Hee-Young Cho) 김덕룡(Deok Ryong Kim) 김욱규(Uk-Kyu Kim) 강희제(Hee-Jea Kang) 김종렬(Jong-Ryoul Kim) 변준호(June-Ho Byun)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제36권 제5호, 341~345쪽, 전체 5쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2010.10.30

영문 초록

Introduction: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. Materials and Methods: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco’s modified Eagle’s medium (DMEM) medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-αwith different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1βwith different concentrations (0.01, 0.1, and 1 ng/mL) were added. Results: Both TNF-αand IL-1βstimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-αand IL-1βincreased the level of ALP expression in a dose-dependent manner. Both TNF-αand IL-1βalso increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. Conclusion: These results suggest that inflammatory cytokines TNF-αand IL-1βcan stimulate the osteoblastic activity of cultured human periosteal-derived cells.

키워드

Periosteal-derived cells Inflammatory cytokine Tumor necrosis factor-α(TNF-α) Interleukin-1β(IL-1β