HPLC-UV를 이용한 혈청 이세파마이신 정량: 약물동태학적 연구

• 영문명: A Simple HPLC-UV Method for the Quantification of Isepamicin in Human Serum: Pharmacokinetic Applications
• 발행기관: 대한약학회
• 저자명: 황은경(Eun-Kyung Hwang) 전성실(Seong-Sill Jeon) 신영희(Young-Hee Shin)
• 간행물 정보: 『약학회지』제59권 제1호 (2015년), 6~11쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2015.02.27

영문 초록

A simple HPLC-UV method was developed and validated for the quantification of human serum isepamicin using phenylisocyanate as a derivatization agent. The linear calibration curve was obtained in the concentration range of 1~100 μg/ml and LLOQ of 1 μg/ml. This method was validated with selectivity, linearity, precision and accuracy, leading to successful application for estimating the pharmacokinetic parameters of isepamicin in Korean healthy subjects following IV infusion of 800 mg isepamicin. The mean t1/2 of isepamicin was 1.55±0.08 hr.

키워드

isepamicin phenylisocyanate derivatization HPLC-UV human serum pharmacokinetics