꾸지뽕나무 추출물의 비만세포 억제에 의한 항알레르기 효과 및 기전

• 영문명: Cudrania tricuspidata Suppresses Mast Cell-Mediated Allergic Response In Vitro and In Vivo
• 발행기관: 대한약학회
• 저자명: 김영미(Young Mi Kim)
• 간행물 정보: 『약학회지』제56권 제1호 (2012년), 26~34쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.02.28

영문 초록

Mast cells play an important role in early and late phase allergic reactions through allergen and IgE-dependent release of histamine, proteases, prostaglandins, and several multifunctional cytokines. In this study, we investigated whether Cudrania tricuspidata extract (CTE) suppresses IgE-mediated allergic responses in mast cells, an allergic animal model, and its mechanism of action in mast cells. We found that CTE inhibited IgE-mediated degranulation and cytokine production in rat basophilic leukemia (RBL)-2H3 mast cells and bone marrow–derived mast cells (BMMC), as well as passive cutaneous anaphylaxis (PCA) in mice. With regard to its mechanism of action, CTE suppressed the activating phosphorylation of spleen tyrosine kinase (Syk), a key enzyme in mast cell signaling processes and that of LAT, a downstream adaptor molecule of Syk in FcεRI-mediated signal pathways. CTE also suppressed the activating phosphorylation of mitogen-activated protein (MAP) kinases and Akt. The present results strongly suggest that the anti-allergic activity of CTE is mediated through inhibiting degranulation and allergic cytokine secretion by inhibition of Syk kinase in mast cells. Therefore, CTE may be useful for the treatment of allergic diseases.

키워드

Cudrania tricuspidata extract (CTE) mast cells allergy src-family kinases syk passive cutaneous anaphylaxis (PCA)