인간 간암 세포주인 SK-Hep-1에서 Stearic Acid에 의한 산화적 스트레스 및 항산화효소의 발현변화 평가

• 영문명: Evaluation of Oxidative Stress and Antioxidant Enzyme Expression in Human Hepatocarcinoma SK-Hep-1 Cells Treated with Stearic Acid
• 발행기관: 대한약학회
• 저자명: 오정민(Jung-Min Oh) 이지윤(Ji-Yoon, Lee) 이관호(Gwan-Ho Lee) 김봉희(Bong-Hee Kim) 김상겸(Sang-Kyum Kim)
• 간행물 정보: 『약학회지』제56권 제1호 (2012년), 14~19쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.02.28

영문 초록

Nonalcoholic steatohepatitis(NASH) is serious metabolic disease related to fatty acid. According to "two hit theory", fatty acid-induced oxidative stress is important factor to progress nonalcoholic steatohepatitis from steatosis. In this study, we evaluated stearic acid induced oxidative stress in human hepatocarcinoma SK-Hep-1 cell. Cell viability, reactive oxygen species (ROS) production, glutathione (GSH), malondialdehyde and expression of antioxidant enzymes were determined at various time-points and concentrations of stearic acid. At 0.2 mM, non-toxic concentration, of stearic acid, production of ROS was significantly increased at 24 hours and the level of GSH was significantly decreased. Expression of superoxide dismutase-1 and 2 was slightly increased in 0.2 mM stearic acid at 24 hours. These results represent that the non-toxic concentration of stearic acid resulted in oxidative stress, suggesting that stearic acid may play a critical role in development of steatohepatitis.

키워드

NASH oxidative stress stearic acid steatosis saturated fatty acid