유전자염기서열 분석에 의해 확진된 뷰베리아 바시아나 각막염

• 영문명: A Case of Beauveria Bassiana Keratitis Confirmed by Gene Sequencing
• 발행기관: 대한안과학회
• 저자명: 전종화 류남희 김의종 장성동,Jong Hwa Jun, MD, Nam Hee Ryoo, MD, PhD, Eui Chong Kim, MD, PhD, Sung Dong Chang, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume55,number6, 923~927쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.06.15

영문 초록

Purpose: To report a case of Beauveria bassiana keratitis that was confirmed by gene sequencing. Case summary: A 70-year-old man presented to our hospital with complaints of ocular pain and deterioration of the visual acuity in his left eye after injury caused by a wood branch one week previously. Visual acuity in the left eye was 20/400 at the time of the first visit. Slit lamp examination showed a central 0.7 × 2.5-mm-sized epithelial defect surrounded by cellular infiltration in the stroma. Scraping of the corneal lesion for microbiological examinations was performed. Initial Gram stain, potassium hydroxide (KOH) mount, and culture were negative. However, fungal hyphae were observed on a KOH mount of the repeated corneal scraping specimen, and a Beauveria species was suspected based on the culture. Beauveria bassiana was confirmed using a MicroSEQ^{® D2 large-subunit ribosomal DNA fungal sequencing kit. Natamycin eye solution was initially instilled bihourly (every two hours), but the persistent epithelial defect and progressive stromal melting finally resulted in a descemetocele. Temporary and permanent amniotic membrane transplantations were performed, and amphotericin B eye solution was administered bihourly (every two hours). The ulcerous lesion gradually improved with no evidence of recurrence. Conclusions: Recently, cases of fungal keratitis have been increasing. Therefore, molecular diagnosis methods such as gene sequencing can be helpful in diagnosis and in improving the prognosis of fungal keratitis caused by rare fungi, as we found in this case. J Korean Ophthalmol Soc 2014;55(6):923-927}

키워드

Beauveria bassiana, Gene sequencing, Keratitis