미세표면분석 기법을 이용한 미용 콘택트렌즈와 일반 콘택트렌즈의 표면거칠기와 세균부착성 비교

• 영문명: Comparison of Surface Roughness and Bacterial Adhesion between Cosmetic Contact Lenses and Conventional Contact Lenses
• 발행기관: 대한안과학회
• 저자명: 지용우 홍순호 정동룡 김응권 이형근,Yong Woo Ji, MD, Soon Ho Hong, Dong Yong Chung, Eung Kweon Kim, MD,Hyung Keun Lee, MD,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume55,number5, 646~655쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.05.15

영문 초록

Purpose: To determine the surface roughness of cosmetic and conventional contact lenses (CLs) and their susceptibility to bacterial adhesion. Methods: Concave surface roughness of cosmetic and conventional hydrogel (Etafilcon A) CLs was measured by atomic force microscopy (AFM) and scanning electron microscopy (SEM). In particular, the surface of the color tinted area of cosmetic CLs was measured. CLs were immersed into a bacterial solution of Pseudomonas aeruginosa for 1, 12, or 24 hours and culture of P. aeruginosa that had adhered to the CLs was performed. Results: Concave surface roughness of cosmetic CLs significantly increased compared with conventional CLs by AFM (p < 0.05). Bacterial colony formation of P. aeruginosa adhering to cosmetic CLs within one hour significantly increased compared with conventional CLs (p = 0.047). Adhesions of P. aeruginosa to CLs within one hour was found to correlate significantly with the surface roughness of CL (r > 0.9, p < 0.05). By SEM, P. aeruginosa had adhered to the color-tinted area more than to the non-color-tinted area of cosmetic CLs. Conclusions: Surface of cosmetic CLs was significantly rougher and initial adhesion of bacteria was higher to cosmetic CLs than to conventional CLs. In particular, an increased number of bacteria was found to be adhered to the color-tinted area of cosmetic CLs. Initial bacterial adhesion is important because it is the first stage of bacterial attachment process to any surface. After then, the adherent bacteria can progress to form a biofilm. Increased surface roughness of CLs contributes to opportunities for the CL to come into contact with bacteria, and thus, initial bacterial adhesion increases. In this study, it is clear that cosmetic CLs are more vulnerable to bacterial adhesion. To avoid serious complications, such as bacterial keratitis, the manufacturing process for smoothing and treating the surface in order to inhibit bacterial adhesion should be developed in the future. J Korean Ophthalmol Soc 2014;55(5):646-655