로테프레드놀과 프레드니솔론 점안제가 테논낭 섬유아세포의 증식에 미치는 영향

• 영문명: Effects of Prednisolone and Loteprednol Eyedrops on the Proliferation of Human Tenon’s Capsule Fibroblasts
• 발행기관: 대한안과학회
• 저자명: 강선희 김재우,Sun Hee Kang, MD, Jae Woo Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume54,number9, 1423~1428쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2013.09.15

영문 초록

Purpose: To compare the effect of loteprednol etabonate (LE) with prednisolone acetate (PDA) drops on the proliferation of human Tenon’s capsule fibroblasts (HTFBs). Methods: Primarily cultured HTFBs were treated with serially diluted PDA and LE for 3 days. Cellular survival was determined by a rapid colorimetric assay using MTT. RT-PCR was performed to determine the relative expression of TGF-β mRNA in response to LE and PDA. Results: PDA inhibited proliferation of HTCF in a dose-dependent manner and LE inhibited significantly the proliferation of HTCF at the higher concentration of 50 μg/ml (p < 0.05). Compared to LE, PDA inhibited proliferation of HTCF significantly at each diluted concentration (p < 0.05). Expressions of TGF-β were decreased as the concentration of both PDA and LE increased. PDA decreased expression of TGF-β more significantly compared to LE at each concentration (p < 0.05). Conclusions: Although LE has offered promising anti-inflammatory efficacy with decreased impact on intraocular pressure, LE may be less effective than PDA in inhibiting fibroblast proliferation and may be not comparable to PDA in preventing excessive scarring after glaucoma filtering surgery.

키워드

Fibroblasts, Loteprednol etabonate, Prednisolone acetate, Proliferation, TGF-β