약물 유도 망막변성 생쥐 모델에서 안구내 면역세포에서의 F4/80와 nestin 발현 양상

• 영문명: Expression Profiles of F4/80 and Nestin in Ocular Immune Cells Following Pharmaceutically Induced Retinal Degeneration in Adult Mice
• 발행기관: 대한안과학회
• 저자명: 안상일 온영훈 박태관,Sang Il Ahn, MD, Young Hoon Ohn, MD, PhD, Tae Kwann Park, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume54,number6, 945~953쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2013.06.15

영문 초록

Purpose: To evaluate the expression patterns of F4/80 and nestin in the ciliary body and the optic nerve following N-methyl- N-nitrosourea (NMU)-induced retinal degeneration in adult mice. Methods: After intraperitoneal injection of MNU (60 mg/kg) in adult mice, the eyes were enucleated at 2, 4, 7 and 30 days. Hematoxylin and eosin (H&E) stain, terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stain and immunohistochemical stains of F/80 and nestin were performed. Results: After MNU treatment, the photoreceptors were destroyed by cell apoptosis. According to immunohistochemistry, F4/80 and nestin were not co-expressed in the control group, but F4/80 was expressed within the ciliary body and optic nerve in the MNU-treated group; the expression of nestin also increased. In the outer nuclear layer, F4/80 and nestin co-expressing cells were observed. Conclusions: In response to retinal damage, the F4/80 and nestin co-expressing cells migrated to the retina from the ciliary body and optic nerve and were activated.

키워드

F4/80, MNU, Nestin, Retinal degeneration