일산화질소가 R28 세포의 생존에 미치는 영향

• 영문명: Effect of Nitric Oxide on the Survival of R28 Cells
• 발행기관: 대한안과학회
• 저자명: 이정일 김재우,Jeong Il Lee, MD, Jae Woo Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume50,number6, 919~922쪽, 전체 4쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.06.15

영문 초록

Purpose: To evaluate the effect of nitric oxide (NO) on the survival of R28 cells. Methods: After immunostaining for GFAP, vimentin, S-100, and neurofilament, R28 cells were exposed to S-nitroso-N-acetyl-D, L-penicillamine (SNAP) at various concentrations, with and without the NO inhibitor, Nω-Nitro-L-arginine methyl ester (L-NAME) for 1 and 3 days. Cellular survival of R28 cells and the production of NO were quantified by rapid colorimetric assays using the MTT and Griess assay, respectively. To evaluate the effect of serum, 10% serum or serum-free media were used separately. Results: R28 cells showed strong immunoreactivity to GFAP and vimentin compared to S-100 or neurofilament. SNAP inhibited the survival of R28 cells in a dose-dependent manner, and this effect of NO on the cellular survival was abolished by L-NAME. These results were similar after exposure for 1 and 3 days, regardless of the presence of serum in the media. Conclusions: The current results suggest that NO decreased the survival of R28 cells. Further studies are necessary to evaluate the mechanism of cytotoxicity of the R28 cells.

키워드

Neurotoxicity, Nitric oxide, R28 cells