일산화질소가 섬유주세포에 의한 교원질 겔의 수축에 미치는 영향

• 영문명: Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels
• 발행기관: 대한안과학회
• 저자명: 김재우.장우석.이수윤,Jae Woo Kim, M.D., Woo Seok Chang, M.D., Su Yoon Lee, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume48,number11, 1548~1553쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.11.30

영문 초록

Purpose: To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs). Methods: After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively. Results: Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05). Conclusions: NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.

키워드

Collagen gel, Contraction, Nitric oxide, Trabecular meshwork