항녹내장 약제가 배양된 사람 결막상피세포에 미치는 영향

• 영문명: The Effect of Antiglaucoma Medication on Cultured Human Conjunctival Epithelial Cells
• 발행기관: 대한안과학회
• 저자명: 이정규 류양환,Jeong Kyu Lee, M.D., Yang Hwan Ryu
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number11, 1811~1818쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.11.30

영문 초록

Purpose: To investigate the toxicity of a short-term application of timolol maleate, dorzolamide, and benzalkonium chloride (BAC) on human conjunctival epithelial cells in vitro. Methods: Chang‘s conjunctival epithelial cell line was treated for 5 min, 15 min, 30 min, and 60 min with various concentrations of timolol, dorzloamide, or BAC, and then examined 4 hrs or 24 hrs later. Cell viabilities were assessed by MTT assay. The expressions of various cytokines by timolol maleate, dorzolamide, and BAC treatment in human conjunctival epithelial cells were evaluated using ELISPOT. Results: BAC significantly decreased survival of conjunctival epithelial cells in a dose and time dependent manner compared with timolol and dorzolamide. Inflammatory cytokines, IL-6 and IL-8, were highly expressed in conjunctival epithelial cells treated with timolol, dorzolamide, and BAC. Conclusions: The present study suggests that increased expression of inflammatory markers, IL-6 and IL-8 might explain the ocular surface disorder in patients receiving antiglaucoma medication.

키워드

Benzalkonium chloride (BAC), Conjunctival epithelial cells, Cytokine, Dorzolamide, Timolol