섬모체 신경조직과 동시 배양된 단순포진바이러스의 축삭이동

• 영문명: Axoplasmic Transport of Herpes Simplex Virus Co-Cultured with Ciliary Nerve
• 발행기관: 대한안과학회
• 저자명: 강석원 고명규,Suk-Won Kang, M.D., Myung-Kyoo Ko, M.D., PhD.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume46,number9, 1575~1581쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2005.09.30

영문 초록

Purpose: To investigate the replication of HSV within cultured cell and axonal transport of HSV within the axon of the ciliary nerve following the injection of HSV into a cultured ciliary nerve. Methods: The explant of the ciliary nerve was cultured with a medium containing nerve growth factor for 30 days when the suspension of HSV-1 (Kos strain) was introduced into the culture dish to co-culture with the ciliary nerve. The ciliary nerve was examined with transmission electron microscopy 30 days after culture and 6 days after co-culture with HSV. Results: The ultrastructure of the explant of the ciliary nerve co-cultured with HSV showed that the viral capsid acquired a viral envelope and viral core, and a capsid and inclusion body within the nucleus. The enveloped virus was scattered within the vesicles of the cytoplasm. The virus-like particles were identified at the axonal fibers. Conclusions: The co-culture of the explant of the ciliary nerve and HSV showed the replicative process of the HSV within the cultured cell. The virus-like particles within the axon showed the evidence axonal transport of the virus under culture conditions.

키워드

Axonal transport, Ciliary nerve, Culture, Herpes simplex virus, Ultrastructure