GC/MS를 이용한 소변 중 대마 대사체 분석의 측정불확도 평가

• 영문명: Uncertainty Evaluation of the Analysis of 11-Nor-9-carboxy-Δ9-tetrahydrocannabinol in Human Urine by GC/MS
• 발행기관: 대한약학회
• 저자명: 김진영(Jin Young Kim) 정재철(Jae Chul Jeong) 서승일(SungIll Suh) 서용준(Yong Jun Suh) 이정직(Jeong Jik Lee) 김종상(Jong Sang Kim) 인문교(Moon Kyo In)
• 간행물 정보: 『약학회지』제52권 제6호 (2008년), 480~487쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.12.31

영문 초록

We described an estimation of measurement uncertainty in quantitative analysis of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCCOOH), the major metabolite of Δ9-tetrahydrocannabinol, in urine sample by solid-phase extraction (SPE) and GC/MS detection. The analytical results were compared and the different contributions to the uncertainty were evaluated. Inter-day and inter-person validation were performed using statistical analysis of several indicative factors. Measurement uncertainty associated with target analyte in real forensic samples was estimated using quality control (QC) data. Traceability of measurement was established through traceable standards, calibrated volumetric glassware and volume measuring device. The major factors of contribution to combined standard uncertainty, were calibration linearity, inter-day repeatability and inter-person reproducibility, while those associated with preparation of analytical standards and sampling volume were not so important considering the degree of contribution. Relative combined standard uncertainties associated with the described method was 12.05% for THCCOOH.

키워드

uncertainty of measurement urinanalysis GC/MS 11-nor-9-carboxy-delta 9-tetrahydrocannabinol