Raw 264.7 세포에서 유해산소 생성에 미치는 Caffeic Acid의 영향

• 영문명: Effect of Caffeic Acid on the Production of Reactive Oxygen Species in Raw 264.7 Cells
• 발행기관: 대한약학회
• 저자명: 최병철(Byung-Chul Choi)
• 간행물 정보: 『약학회지』제52권 제6호 (2008년), 441~445쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.12.31

영문 초록

To investigate effect of caffeic acid on the intracellular reactive oxygen species production, we used DHE for intracellular superoxide anion production, DCF for intracellular H2O2 production and DHR for intracellular hydroperoxide production in Raw 264.7 cells. DPPH assay showed that antioxidant activity of caffeic acid with 39.5 μM of IC50 values was similar to that of ascorbic acid with 41.3 μM of IC50 values. Caffeic acid dose-dependently inhibited silica-induced H2O2 and hydroperoxide production but did not affect superoxide anion production in Raw 264.7 cells, which suggest that antioxidant effect of caffeic acid acts on the post-step of superoxide anion. On the other hand, caffeic acid showed a potent antioxidant effect in lCuSO4-induced lipid peroxidation. Furthermore, plasma superoxide dismutase activity (3.43±0.23 U/ml) in 10 mg/kg caffeic acid-fed mice was significantly higher than that (2.32±0.24 U/ml) of control. From the above results, it is referred that caffeic acid appears to have potent anti-oxidant activity in both cell system and in vivo system.

키워드

caffeic acid reactive oxygen species nitric oxide superoxide dismutase