Serratia속의 신균주가 생산하는 단백질분해효소

• 영문명: Studies on proteolytic enzyme from A New Strain in Serratia sp.
• 발행기관: 대한약학회
• 저자명: 최완수(Wahn Soo Choi) 정계종(Kae Jong Chung) 이주경(Joo Kyung Lee) 박주웅(Joo Woong Park) 이상훈(Sang Hoon Lee) 이진복(Jin Bok Lee) 이송락(Song Rag Lee) 최신원(Shin Won Choi)
• 간행물 정보: 『약학회지』제37권 제2호 (1993년), 129~135쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.04.30

영문 초록

Serratia sp. Y-4 was isolated from soil. Many characteristics of the strain and optimum cultivation condition for protease production were investigated. The protease from Serratia sp. Y-4 was purified and studied for the properties of the enzyme. The isolated strain was identified to the genus Serratia. The strain was cultivated in 1%-casein, 0.5%-Na3PO4.7H2O, 0.1%-NaCl, 0.05%-KCl, 0.02%-MgSO4.7H2O, 0.02%-CaCl2.2H2O, 0.02%-ZnSO4.7H2O, 0.02%-MnCl2.4H2O and 0.5%-soy bean oil at pH 7.0 for 35 hrs. The enzyme was purified about 5.89 fold from the culture broth with 31.1% recovery and 19,613mc/mg through ultrafiltration, ammonium sulfate, DEAE-sephacel and Superose-12 chromatography. The purified enzyme was identified as one band by isoelectric focusing, SDS- and native-PAGE. It has maxium activity at 37oC and pH 9.0. Molecular weight of it is approx. 50 kD and pI is about 6.70. Its Km value for casein was 20mg/ml. 5 mM-EDTA, 5 mM-SDS, Ag+1, Cu+2, Hg+2 and Pb+2 inhibited the enzyme.

키워드

Serratia sp. Y-4 Proteolytic enzyme cultivation purification.