Murrayafoline-A에 의한 심실 근육세포 Ca2+

• 영문명: Enhancement of Ca 2+ in Rat Ventricular Myocytes
• 발행기관: 대한약학회
• 저자명: 김준철 뉘엔 만 콩 우선희
• 간행물 정보: 『약학회지』제58권 제4호 (2014년), 245~249쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.08.31

영문 초록

Murrayafoline-A (1-methoxy-3-methylcarbazole) is a monomeric carbazole alkaloid found in Murraya euchres- tifolia HAYATA and Glycosmis stenocarpa. We have recently shown that murrayafoline-A has positive inotropic effect in iso-lated rat ventricular myocytes. To know possible mechanisms for the positive inotropic effect of murrayafoline-A we examined the effects of murrayafoline-A on in situ behavior of cardiac Ca 2+ release units (‘Ca2+ sparks’) and sarcoplasmic reticulum (SR) Ca 2+ loading using confocal Ca 2+ imaging method in single rat ventricular myocytes. Murrayafoline-A sig-nificantly increased the frequency (events/(103μm2· s)) of Ca 2+sparks in a concentration-ndent manner, with an EC50 of 28±6.4 μM and a maximal ~twofold change. The Ca 2+ content in the SR, measured as caffeine (10 mM)-induced Ca 2+transient, was significantly increased by murrayafoline-A (≈116% and ≈123% of control at 25 and 100 μM, respectively). In addition, murrayafoline-A significantly increased the fractional Ca 2+ release, suggesting increase in the efficacy of Ca 2+ release at given SR Ca 2+ loading. These results suggest that murrayafoline-A may enhance contractility via increase in Ca 2+ release from the SR through the ryanodine receptors in ventricular myocytes.

목차

실험방법

실험결과 및 고찰

결 론

감사의 말씀

References

키워드

murrayafoline-A, Ca 2+ spark ventricular myocytes SR Ca 2+ content fractional Ca 2+ release